The genomic environments of six unrelated variant surface glycoprotein (VSG) gene families in the IsTaR 1 serodeme of Trypanosoma brucei were examined by Southern blot analysis of DNA resolved by conventional and pulse field gradient electrophoresis. The genomic locations of these gene family members were characterized in variants arising from 19 independent antigenic switches of which 13 represent single relapses. We have identified 9 distinct telomeric sites containing VSG genes on 7 different chromosomes ranging in size from approximately 65 kilobase pairs to greater than 3 megabase pairs. VSG genes are expressed in at least 6 of these telomeric sites. All of the observed antigenic switches can be explained by two types of processes: gene conversion and telomeric activation, with almost half (9/19) the result of combinations of these processes. The implications of these observations are discussed with particular reference to the frequencies of occurrence of each process.