This core provides the tools for capturing and analyzing images, whether they come from cells viewed through a microscope or from molecules identified on gels and blots. The fluorescence microscopy and quantitative autoradiography imaging equipment allows researchers to describe cell changes and function under varying conditions and to characterize molecules following separation on gels.  The facility is overseen by a Scientific Advisor, Dr. Amy DeRocher.

Imaging Equipment

• Applied Precision DeltaVision RT optical/digital-sectioning microscope: For optimal 3D live-cell imaging in real time. This specialized instrument is equipped with DIC optics for visible imaging and up to six channels (DAPI, FITC, Texas Red, CFP, YFP and Cy-5) for capturing fluorescently labeled cells. This system is capable of multi-wavelength automated image capture. The SoftWorx software package comes with deconvolution capabilities for the highest quality images.
• Nikon Eclipse E600 upright microscope: This scope has phase contrast optics and three filters for fluorescence imaging (FITC, DAPI and Texas Red).  It is equipped with both a high speed Cool Snap CCD camera used for fluorescence and phase contrast images and a true color camera used for chromatic staining in tissue samples. Metamorph analysis software is included.
• Nikon TE2000U inverted microscope: This scope has phase contrast and DIC optics for visible imaging, three filters for fluorescence imaging (FITC, DAPI and Texas Red), a motorized stage and a high speed Cool Snap CCD camera. This system is capable of single wavelength automated image capture. There are stage inserts for viewing slides, Petri dishes, chamber slides, multi-well plates and tissue culture flasks. Metamorph analysis software is included.
• Odyssey Imaging System:  For one- and two-color Western blots, in-gel fluorescence detection (acrylamide and agarose gels), quantitative fluorescence detection in standard microtiter plates (in-cell western), immunohistochemistry of tissue sections and general imaging of coomassie stained gels and membranes. 
• Storm 860 phosphorimager: This instrument is capable of scanning a variety of radioactive and chemifluorescent signals from gels, blots and phosphor screens.

Some equipment may be available for external use.  To get more information or to discuss your project, please contact the Imaging Core Manager, Tracy Mitchell at (206) 256-7498.