Seattle BioMed

House Primers

The T7(s), T3, SP6, M13 (-21) Forward, and the M13 Reverse primers are provided by the Seattle BioMed sequencing facility at no additional cost. Please indicate in the comment section of the online request form if you have added your own standard primers.

Primer Sequence
M13 Reverse AAC AGC TAT GAC CAT G
M13(-21) Forward TGT AAA ACG ACG GCC AGT
SP6 GAT TTA GGT GAC ACT ATA G
T3 ATT AAC CCT CAC TAA AGG A
T7 AAT ACG ACT CAC TAT AG
T7 Reverse TAG TTA TTG CTC AGC GGT GG
T7 Terminator GCT AGT TAT TGC TCA GCG G
T7 Uni TAA TAC GAC TCA CTA TAG GG