This core provides high-speed cell sorting, flow cytometer analysis and related consultation services to determine the characteristics of the cells in a complex cell mixture. Cell populations of interest can then be collected on the cell sorter for further experimentation. The facility is overseen by a faculty Scientific Advisor, Dr. Helen Horton.

Flow Cytometry Equipment

• Cytopeia Influx cell sorter: Equipped with three lasers (ultraviolet, blue and red) and capable of detecting eight colors; the sorter is housed in a Biosafety Level 3 (BSL-3) facility and additionally includes a biocontainment system for sorting BSL-2+ level infectious agents.
• BD FACS AriaII cell sorter: Equipped with four lasers (ultraviolet, blue, violet and red) and capable of detecting up to 17 colors simultaneously.  The sorter also has an aerosol management system for sorting BSL-2 infectious agents.  
• BD LSRII analyzer: Equipped with four lasers (violet, blue, green and red) and capable of detecting up to 18 colors; includes a high throughput sampler (HTS) that can rapidly introduce samples from 96 and 384 well plates.
• Beckman Coulter Epics XL-MCL analyzer: Equipped with a blue laser capable of detecting up to four colors; includes automated multi-tube carousel loader (100 tubes/hour).

To get more information or to discuss an upcoming project, please contact the Flow Cytometry Core Manager, Nick Lejarcegui at (206) 256-7164.